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Learn about periodic acid schiff stain (pas staining) technique and discover its applications in disease diagnosis especially in differentiating all and aml. The aim of the present study is to evaluate the role of some cytochemical stains namely mpo, sbb, nse and pas in the diagnosis of acute leukemia in comparison to immunophenotyping using. Learn about pas staining, its protocol, applications in pathology, and troubleshooting tips for accurate results in diagnostic and research settings.

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Periodic acid schiff (pas) stain performed on a bone marrow aspirate smear involved by acute erythroid leukemia (formerly known as pure erythroid leukemia) A total of 25 out of 25 aml cases and 28 out of 32 cases of all were correctly diagnosed based on morphology and cytochemical staining. Note the abnormal (chunky globular) pattern of staining in the erythroblasts.

Monocytes are faintly positive while lymphocytes and red blood cells lack the enzyme

This stain is particularly useful in a setting of acute leukaemia, where positive staining indicates that the leukaemic cells are of myeloid origin. Cytochemical analysis of leukaemia using pas and mpo rendered the diagnosis in 92.9% of acute leukaemia cases in our study